Probing Contaminant-Induced Alterations in Chlorophyll Fluorescence by AC-Dielectrophoresis-Based 2D-Algal Array.
Coralie SiebmanOrlin D VelevVera I SlaveykovaPublished in: Biosensors (2018)
The investigation of contaminant impact on algae requires rapid and reliable cell collection and optical detection. The capability of alternative current (AC) dielectrophoresis (DEP) collection of whole cell arrays with combined fluorescence microscopy detection to follow the alterations of chlorophyll fluorescence during environmental contaminant exposure was explored. The application of an AC-field of 100 V cm-1, 100 Hz for 30 min to capture and immobilize the cells of green alga Chlamydomonas reinhardtii in two-dimensional (2D) arrays does not induce changes in chlorophyll fluorescence. The results demonstrate that DEP-based 2D-arrays allow non-invasive detection of chlorophyll fluorescence change upon exposure to high concentrations of copper oxide nanoparticles and ionic copper. These results were in agreement with data obtained by flow cytometry used as a comparative method. The tool was also applied to follow the effect of a number of ubiquitous contaminants such as inorganic mercury, methylmercury, and diuron. However, a statistically significant short-term effect was observed only for mercury. Overall, DEP-based 2D-arrays of algal cells with fluorescence detection appear to be suitable for stain-free probing the effects on the photosynthetic microorganisms in highly polluted environment.
Keyphrases
- single molecule
- energy transfer
- loop mediated isothermal amplification
- oxide nanoparticles
- induced apoptosis
- label free
- flow cytometry
- high density
- high resolution
- single cell
- quantum dots
- cell cycle arrest
- cell therapy
- mesenchymal stem cells
- bone marrow
- high speed
- stem cells
- cell death
- optical coherence tomography
- heavy metals
- cell proliferation
- ionic liquid
- drinking water
- diabetic rats
- sensitive detection
- high glucose
- endothelial cells