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A bio-inspired approach for the synthesis of few-layer graphene using beetle defensive gland extract.

Anthyalam Parambil AjaykumarK NikhilaOvungal SabiraKodangattil Narayanan JayarajSudhir Rama VarmaV A RasheedV S BinithaKalapparambil SreejaResmi M RamakrishnanAnnet Babu
Published in: RSC advances (2024)
Graphene exhibits remarkable properties and holds substantial promise for diverse applications. Its unique combination of thermal, chemical, physical, and biological properties makes it an appealing material for a wide range of uses. But, the lack of an economical and environmentally friendly approach to synthesize good-quality graphene represents an immense challenge for the scientific community. What makes this research unique is the utilization of the defensive gland extract from the beetle species Luprops tristis (Order: Coleoptera, Family: Tenebrionidae) to synthesize a few layers of graphene (FLG). This innovative incorporation of natural resources and exploration of biologically inspired methods provides an eco-friendly and cost-effective alternative to conventional graphene production techniques. The exfoliated graphene displayed antimicrobial effects against both Gram-positive ( Staphylococcus aureus ) and Gram-negative ( Escherichia coli ) bacteria, with particularly potent bactericidal activity. Additionally, the cytotoxicity assay demonstrated the anti-cancer properties of biosynthesized graphene against Daltons Lymphoma Acetic (DLA) cells.
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