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Competitive Exclusion of Intra-Genus Salmonella in Neonatal Broilers.

Megan Rose PinedaMichael H KogutKenneth GenoveseYuhua Zhang FarnellDan ZhaoXi WangAllison MilbyMorgan Brian Farnell
Published in: Microorganisms (2021)
Salmonellosis is a zoonotic infection caused by Salmonella enterica serotypes contracted from contaminated products. We hypothesized that competitive exclusion between Salmonella serotypes in neonatal broilers would reduce colonization and affect the host immune response. Day of hatch broilers were randomly allocated to one of six treatment groups: (1) control, which received saline, (2) Salmonella Kentucky (SK) only on day 1 (D1), (3) Salmonella Typhimurium (ST) or Salmonella Enteritidis (SE) only on D1, (4) SK on D1 then ST or SE on day 2 (D2), (5) ST or SE on D1 then SK on D2, and (6) SK and ST or SE concurrently on D1. Salmonella gut colonization and incidence were measured from cecal contents. Livers and spleens were combined and macerated to determine systemic translocation. Relative mRNA levels of interleukin-1β (IL-1β), IL-6, IL-10, IL-18, and gamma interferon (IFN-γ) were measured in cecal tonsils and liver to investigate local and systemic immune responses. When a serotype was administered first, it was able to significantly reduce colonization of the following serotype. Significant changes were found in mRNA expression of cytokines. These results suggest competitive exclusion by Salmonella enterica serotypes affect local and systemic immune responses.
Keyphrases
  • immune response
  • listeria monocytogenes
  • escherichia coli
  • dendritic cells
  • heat stress
  • toll like receptor
  • dengue virus
  • klebsiella pneumoniae
  • zika virus
  • drinking water
  • multidrug resistant
  • drug induced