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AIRR Community Guide to Planning and Performing AIRR-Seq Experiments.

Anne EugsterMagnolia L BostickNidhi GuptaEncarnita Mariotti-FerrandizGloria KrausWenzhao MengCinque SotoJohannes TrückUlrik StervboEline T Luning Praknull null
Published in: Methods in molecular biology (Clifton, N.J.) (2022)
The development of high-throughput sequencing of adaptive immune receptor repertoires (AIRR-seq of IG and TR rearrangements) has provided a new frontier for in-depth analysis of the immune system. The last decade has witnessed an explosion in protocols, experimental methodologies, and computational tools. In this chapter, we discuss the major considerations in planning a successful AIRR-seq experiment together with basic strategies for controlling and evaluating the outcome of the experiment. Members of the AIRR Community have authored several chapters in this edition, which cover step-by-step instructions to successfully conduct, analyze, and share an AIRR-seq project.
Keyphrases
  • rna seq
  • genome wide
  • single cell
  • high throughput sequencing
  • mental health
  • healthcare
  • dna methylation
  • optical coherence tomography
  • gene expression
  • binding protein