The CCR4-NOT complex component NOT1 regulates RNA-directed DNA methylation and transcriptional silencing by facilitating Pol IV-dependent siRNA production.
Hao-Ran ZhouRong-Nan LinHuan-Wei HuangLin LiTao CaiJian-Kang ZhuShe ChenXin-Jian HePublished in: The Plant journal : for cell and molecular biology (2020)
Small interfering RNAs (siRNAs) are responsible for establishing and maintaining DNA methylation through the RNA-directed DNA methylation (RdDM) pathway in plants. Although siRNA biogenesis is well known, it is relatively unclear about how the process is regulated. By a forward genetic screen in Arabidopsis thaliana, we identified a mutant defective in NOT1 and demonstrated that NOT1 is required for transcriptional silencing at RdDM target genomic loci. We demonstrated that NOT1 is required for Pol IV-dependent siRNA accumulation and DNA methylation at a subset of RdDM target genomic loci. Furthermore, we revealed that NOT1 is a constituent of a multi-subunit CCR4-NOT deadenylase complex by immunoprecipitation combined with mass spectrometry and demonstrated that the CCR4-NOT components can function as a whole to mediate chromatin silencing. Therefore, our work establishes that the CCR4-NOT complex regulates the biogenesis of Pol IV-dependent siRNAs, and hence facilitates DNA methylation and transcriptional silencing in Arabidopsis.
Keyphrases
- dna methylation
- genome wide
- gene expression
- copy number
- transcription factor
- dendritic cells
- mass spectrometry
- arabidopsis thaliana
- regulatory t cells
- cancer therapy
- immune response
- high resolution
- liquid chromatography
- drug delivery
- single cell
- high throughput
- capillary electrophoresis
- wild type
- cell wall
- plant growth