Templated insertions at VD and DJ junctions create unique B-cell receptors in the healthy B-cell repertoire.
Marvyn T KoningEsther M VletterRik RademakerRochelle D VergroesenIgnis J M TrollmannPaul ParrenCornelis A M van BergenHans U SchererSzymon M KiełbasaRené E M ToesHendrik VeelkenPublished in: European journal of immunology (2020)
RAG complexes recognise (cryptic) RSS sites both in and outside immunoglobulin sites. Excision circles may be reinserted into V(D)J rearrangements as long templated insertions to diversify the adaptive immune repertoire. We show that such VDJ with templated insertions are incidentally found in the repertoire of healthy donors.