Development of a recombinase polymerase amplification combined with a lateral flow dipstick assay for rapid detection of the Mycoplasma bovis.

Guimin ZhaoPeili HouYanjun HuanChengqiang HeHongmei WangHongbing He

Published in: BMC veterinary research (2018)

To the author's knowledge, this is the first report using an RPA-FLD assay to visualise and detect M. bovis. Comparative analysis with qPCR indicates the potential of this assay for rapid diagnosis of bovine mycoplasmosis in resource limited settings.

Keyphrases

high throughput
healthcare
loop mediated isothermal amplification
nucleic acid
risk assessment
climate change