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Comparative Analysis of Begonia Plastid Genomes and Their Utility for Species-Level Phylogenetics.

Nicola HarrisonRichard J HarrisonCatherine A Kidner
Published in: PloS one (2016)
Recent, rapid radiations make species-level phylogenetics difficult to resolve. We used a multiplexed, high-throughput sequencing approach to identify informative genomic regions to resolve phylogenetic relationships at low taxonomic levels in Begonia from a survey of sixteen species. A long-range PCR method was used to generate draft plastid genomes to provide a strong phylogenetic backbone, identify fast evolving regions and provide informative molecular markers for species-level phylogenetic studies in Begonia.
Keyphrases
  • high throughput sequencing
  • genetic diversity
  • gene expression
  • single cell
  • copy number