MnO2 Nanotube-Based NanoSearchlight for Imaging of Multiple MicroRNAs in Live Cells.
Qian LuDaniel EricsonYang SongChengzhou ZhuRanfeng YeSong-Qin LiuJoseph A SpernyakDan DuHe LiYun WuYuehe LinPublished in: ACS applied materials & interfaces (2017)
Sensitive assay and imaging of multiple low-abundance microRNAs (miRNAs) in living cells remain a grand challenge. Herein, based on polyelectrolyte-induced reduction, a facile approach has been proposed to synthesize novel MnO2 nanotubes. Owing to the remarkably strong fluorescence quenching ability, low cytotoxicity, and excellent colloid stability, the as-prepared MnO2 nanotubes showed great potential for simultaneous detection and imaging of multiple miRNAs in vitro and in situ in living cells for the first time. Besides, MnO2 nanotubes can be reduced to Mn2+ by intracellular acid pH or glutathione, which may serve as an activatable contrast reagent for MRI. Therefore, the MnO2 nanotube-based probes, termed "NanoSearchlight", provide a promising, multimodal imaging tool for precise and accurate diagnosis and prognosis of cancers.
Keyphrases
- living cells
- fluorescent probe
- high resolution
- single molecule
- fluorescence imaging
- small molecule
- contrast enhanced
- mass spectrometry
- high throughput
- photodynamic therapy
- chronic pain
- gold nanoparticles
- young adults
- pi k akt
- cell proliferation
- reduced graphene oxide
- drug induced
- endothelial cells
- wastewater treatment