LC-MS/MS profiling of polyphenol-enriched leaf, stem and root extracts of Korean Humulus japonicus Siebold & Zucc and determination of their antioxidant effects.
Jin Young ChoiKebede Taye DestaSoo Jung LeeYun-Hi KimSung Chul ShinGon-Sup KimSung Joong LeeJae-Han ShimAhmet HacımüftüoğluA M Abd El-AtyPublished in: Biomedical chromatography : BMC (2018)
Polyphenols from ethyl acetate extracts from the leaves, stems and roots of Korean Humulus japonicus were comprehensively profiled using liquid chromatography-electrospray ionization-tandem mass spectrometry. A total of 36 polyphenols were detected, of which 26 were structurally characterized based on their [M - H]- peak, tandem mass spectrometry fragmentation pattern, UV-vis absorption and published data. Validation data provided satisfactory results for the evaluated parameters. The determination coefficients were ≥0.9812. The limits of detection and quantification were 0.017-0.573 and 0.056-1.834 mg/L, respectively, indicating good performance limits. The accuracy (expressed as percentage recovery) at 50 and 100 mg/L was 71.4-99.7 and 75.1-105.1%, with precisions (expressed as relative standard deviation) of 1.5-7.3 and 0.8-4.1%, respectively, indicating acceptable accuracy and precision values. The leaves were rich in total polyphenols (3089.9 ± 6.4 mg/kg of fresh sample) followed by the stems (1313.9 ± 6.4 mg/kg of fresh sample) and roots (655.2 ± 2.7 mg/kg of fresh sample). Antioxidant activity, determined by α,α-diphenyl-β-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) scavenging activity and ferric reducing antioxidant power assay, revealed the lowest EC50 value for the leaf extracts, indicating a higher scavenging activity in this tissue followed by the roots and stems. Overall, the results indicated that H. japonicus is rich in polyphenols and could be a potential alternative to Humulus lupulus (hop plant) in the brewery industry.
Keyphrases
- tandem mass spectrometry
- liquid chromatography
- solid phase extraction
- ultra high performance liquid chromatography
- high performance liquid chromatography
- simultaneous determination
- gas chromatography
- mass spectrometry
- high resolution mass spectrometry
- molecularly imprinted
- high resolution
- electronic health record
- oxidative stress
- single cell
- big data
- systematic review
- anti inflammatory
- ionic liquid
- high throughput
- climate change
- essential oil
- cell wall
- meta analyses