Rapid detection of NDM and VIM carbapenemase encoding genes by recombinase polymerase amplification and lateral flow-based detection.
Abdulrahman K S AyfanJoanne MacdonaldPatrick N A HarrisClaire HeneyDavid J PatersonElla TrembizkiClaire Y T WangDavid M WhileyHosam Mamoon ZowawiAdam David IrwinPublished in: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology (2021)
Carbapenemase-producing organisms (CPOs) pose a serious clinical threat and rapid detection tools are essential to aid in patient management. We developed rapid and simple molecular tests to detect blaNDM-type and blaVIM-type carbapenemase genes using recombinase polymerase amplification (RPA) combined with a lateral flow detection. The tests could provide results in approximately 15 min when using DNA extracts, with limits of detection of 9.2 copies/μl for the blaNDM-type assay and 7.5 copies/μl for blaVIM-type assay, and successfully detected all isolates harbouring the carbapenemase encoding genes in a panel of 57 isolates. These RPA tests may be suitable for use in low-resource settings to tailor rapid implementation of infection control precautions and antibiotic stewardship.
Keyphrases
- loop mediated isothermal amplification
- klebsiella pneumoniae
- gram negative
- label free
- multidrug resistant
- acinetobacter baumannii
- genome wide
- real time pcr
- sensitive detection
- escherichia coli
- high throughput
- primary care
- healthcare
- genome wide identification
- nucleic acid
- bioinformatics analysis
- dna methylation
- transcription factor
- pseudomonas aeruginosa
- quantum dots