Control of Biofilm Formation by an Agrobacterium tumefaciens Pterin-Binding Periplasmic Protein Conserved Among Pathogenic Bacteria.
Jennifer L GreenwichJustin L EaganNathan FeirerKaleb BoswinkleGeorge MinasovLudmilla ShuvalovaNicole L InnissJakka RaghavaiahArun K GhoshKarla J F SatchellKylie D AllenClay FuquaPublished in: bioRxiv : the preprint server for biology (2023)
Biofilms are bacterial communities attached to surfaces, physiologically distinct from free-living cells, and a common cause of persistent infections. Here we define the mechanism of a novel biofilm regulatory system based on excreted metabolites called pterins, that is conserved within a wide range of Gram-negative bacteria, including multiple pathogens of animals and plants. The molecular mechanism of pterin-dependent regulation is reported including structural determination of several members of a new family of pterin-binding proteins. Pterins are produced across all domains of life and mechanistic insights into this regulatory circuit could lead to new advances in antibiofilm treatments.
Keyphrases
- biofilm formation
- living cells
- candida albicans
- transcription factor
- pseudomonas aeruginosa
- fluorescent probe
- staphylococcus aureus
- dna binding
- escherichia coli
- single molecule
- binding protein
- ms ms
- antimicrobial resistance
- cystic fibrosis
- solid phase extraction
- gram negative
- small molecule
- amino acid
- multidrug resistant
- mass spectrometry