Noninvasive imaging of amyloid-β (Aβ) species in vivo is important for the early diagnosis of Alzheimer's disease (AD). In this paper, we report a near-infrared (NIR) fluorescence (FL) and positron emission tomography (PET) bimodal probe (NIR-[ 68 Ga]) for in vivo imaging of both soluble and insoluble Aβ species. NIR-[ 68 Ga] holds a high binding affinity, high selectivity and high sensitivity toward Aβ 42 monomers, oligomers, and aggregates in vitro . In vivo imaging results show that NIR-[ 68 Ga] can cross the blood-brain-barrier (BBB), and produce significantly higher PET and NIR FL bimodal signals in the brains of APP/PS1 transgenic AD mice relative to that of age-matched wild-type mice, which are also validated by the ex vivo autoradiography and histological staining images. Our results demonstrate that NIR-[ 68 Ga] is an efficient NIR FL and PET bimodal probe for the sensitive imaging of soluble and insoluble Aβ species in AD mice.
Keyphrases
- pet ct
- positron emission tomography
- fluorescence imaging
- computed tomography
- photodynamic therapy
- fluorescent probe
- drug release
- wild type
- high resolution
- living cells
- pet imaging
- high fat diet induced
- blood brain barrier
- skeletal muscle
- single molecule
- adipose tissue
- quantum dots
- drug delivery
- machine learning
- insulin resistance
- metabolic syndrome
- mild cognitive impairment
- energy transfer