Induced pluripotent stem cell-derived myeloid cells expressing OX40 ligand amplify antigen-specific T cells in advanced melanoma.
Toshihiro KimuraSatoshi FukushimaEtsuko OkadaHaruka KuriyamaHisashi KanemaruMina Kadohisa-TsurutaYosuke KuboSatoshi NakaharaAki TokuzumiIkko KajiharaKatsunari MakinoAzusa MiyashitaJun AoiTakamitsu MakinoHirotake TsukamotoYasuharu NishimuraTakashi InozumeRong ZhangYasushi UemuraSatoru SenjuHironobu IhnPublished in: Pigment cell & melanoma research (2020)
Immune checkpoint inhibitors improved the survival rate of patients with unresectable melanoma. However, some patients do not respond, and variable immune-related adverse events have been reported. Therefore, more effective and antigen-specific immune therapies are urgently needed. We previously reported the efficacy of an immune cell therapy with immortalized myeloid cells derived from induced pluripotent stem cells (iPS-ML). In this study, we generated OX40L-overexpressing iPS-ML (iPS-ML-Zsgreen-OX40L) and investigated their characteristics and in vivo efficacy against mouse melanoma. We found that iPS-ML-Zsgreen-OX40L suppressed the progression of B16-BL6 melanoma, and prolonged survival of mice with ovalbumin (OVA)-expressing B16 melanoma (MO4). The number of antigen-specific CD8+ T cells was higher in spleen cells treated with OVA peptide-pulsed iPS-ML-Zsgreen-OX40L than in those without OX40L. The OVA peptide-pulsed iPS-ML-Zsgreen-OX40L significantly increased the number of tumor-infiltrating T lymphocytes (TILs) in MO4 tumor. Flow cytometry showed decreased regulatory T cells but increased effector and effector memory T cells among the TILs. Although we plan to use allogeneic iPS-ML in the clinical applications, iPS-ML showed the tumorgenicity in the syngeneic mice model. Incorporating the suicide gene is necessary to ensure the safety in the future study. Collectively, these results indicate that iPS-ML-Zsgreen-OX40L therapy might be a new method for antigen-specific cancer immunotherapy.
Keyphrases
- regulatory t cells
- induced apoptosis
- dendritic cells
- cell therapy
- cell cycle arrest
- low density lipoprotein
- bone marrow
- flow cytometry
- end stage renal disease
- stem cells
- newly diagnosed
- skin cancer
- chronic kidney disease
- transcription factor
- acute myeloid leukemia
- ejection fraction
- type diabetes
- radiation therapy
- metabolic syndrome
- high fat diet induced
- genome wide
- low dose
- type iii
- insulin resistance
- skeletal muscle
- peritoneal dialysis
- adipose tissue
- mass spectrometry
- copy number
- basal cell carcinoma
- stress induced
- wild type