An "off-on" electrochemiluminescence aptasensor for microcystin-LR assay based on the resonance energy transfer from PTCA/NH2-MIL-125(Ti) to gold nanoparticles.
Jingxian LiDing JiangXueling ShanWenchang WangZhidong ChenPublished in: Mikrochimica acta (2020)
A novel "off-on" electrochemiluminescence (ECL) aptasensor based on ECL resonance energy transfer (RET) system from 3,4,9,10-perylenetetracar-boxylic acid/the metal-organic frameworks NH2-MIL-125(Ti) (PTCA/NH2-MIL-125) to Au nanoparticles (Au NPs) was put forward for microcystin-LR (MC-LR) assay. The electrochemiluminescence (ECL) emission spectra of PTCA/NH2-MIL-125 show excellent spectral overlap with the UV-vis absorption spectrum of Au NPs. A linear response ranging from 10 fM (9.95 × 10-6 μg L-1) to 0.1 μM (99.5 μg L-1) was obtained, and the detection limit was 3.6 fM (3.58 × 10-6 μg L-1) under optimal conditions at a potential of - 1.6 V (S/N = 3). Besides, the prepared ECL-RET MC-LR aptasensor exhibited good selectivity and stability. Graphical abstract.
Keyphrases
- energy transfer
- metal organic framework
- sensitive detection
- quantum dots
- gold nanoparticles
- reduced graphene oxide
- room temperature
- loop mediated isothermal amplification
- high throughput
- perovskite solar cells
- optical coherence tomography
- solid phase extraction
- magnetic resonance
- density functional theory
- computed tomography
- visible light
- climate change
- real time pcr