N-Heterocyclic Carbene-Gold(I) Complexes Conjugated to a Leukemia-Specific DNA Aptamer for Targeted Drug Delivery.
Weijia NiuXigao ChenWeihong TanAdam S VeigePublished in: Angewandte Chemie (International ed. in English) (2016)
This report describes the synthesis and characterization of novel N-heterocyclic carbene (NHC)-gold(I) complexes and their bioconjugation to the CCRF-CEM-leukemia-specific aptamer sgc8c. Successful bioconjugation was confirmed by the use of fluorescent tags on both the NHC-Au(I) complex and the aptamer. Cell-viability assays indicated that the NHC-Au(I) -aptamer conjugate was more cytotoxic than the NHC-gold complex alone. A combination of flow cytometry, confocal microscopy, and cell-viability assays provided clear evidence that the NHC-Au(I) -aptamer conjugate was selective for targeted CCRF-CEM leukemia cells.
Keyphrases
- sensitive detection
- quantum dots
- cancer therapy
- gold nanoparticles
- drug delivery
- flow cytometry
- label free
- acute myeloid leukemia
- magnetic nanoparticles
- bone marrow
- reduced graphene oxide
- high throughput
- induced apoptosis
- silver nanoparticles
- single molecule
- cell free
- cell cycle arrest
- living cells
- cell proliferation