Characterization of RHD alleles present in serologically RHD-negative donors determined by a sensitive microplate technique.
Tatiane Aparecida de Paula VendrameCarine Prisco ArnoniJanaína Guilhem MunizRosangela de Medeiros PersonAfonso José Pereira CortezFlavia Roche Moreira LatiniLilian CastilhoPublished in: Vox sanguinis (2019)
No weak D type was found in either screening populations. Additionally, 353 (18·4%) D-negative samples presented previously reported non-functional RHD genes, 2 samples had a DEL allele, and 6 samples demonstrated new alleles, including one novel DEL allele. Our study identified six new RHD alleles and showed that the inclusion of a confirmatory test using serological methodology with high sensitivity can reduce the frequency of weak D samples typed as D-negative.