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Non-reproducible sequence artifacts in FFPE tissue: an experience report.

Richard OfnerCathrin RitterSelma UgurelLorenzo CerroniMathias StillerThomas BogenriederFlavio SolcaDavid SchramaJürgen Christian Becker
Published in: Journal of cancer research and clinical oncology (2017)
Since DNA from FFPE tissue is usually the primary source for mutational analyses, appropriate measures must be implemented in the workflow to assess DNA damage in formalin-fixed tissue to ensure accurate detection of actionable mutations and minimize the occurrence of sequence artifacts.
Keyphrases
  • dna damage
  • risk assessment
  • oxidative stress
  • high resolution
  • magnetic resonance
  • magnetic resonance imaging
  • image quality
  • cell free
  • computed tomography
  • real time pcr