G-Quadruplex/Hemin-Mediated Polarity-Switchable and Photocurrent-Amplified System for Escherichia coli O157:H7 Detection.
Rui GeShu-Min ZhangHan-Jie DaiJie WeiTian-Hui JiaoQing-Min ChenQuan-Sheng ChenXiao-Mei ChenPublished in: Journal of agricultural and food chemistry (2023)
The contamination of food by pathogens is a serious problem in global food safety, and current methods of detection are costly, time-consuming, and cumbersome. Therefore, it is necessary to develop rapid, portable, and sensitive assays for foodborne pathogens. In addition, assays for foodborne pathogens must be resistant to interference resulting from the complex food matrix to prevent false positives and negatives. In this study, hemin and reduced graphene oxide-MoS 2 sheets (GMS) were used to design a near-infrared (NIR)-responsive photoelectrochemical (PEC) aptasensor with target-induced photocurrent polarity switching based on a hairpin aptamer (Hp) with a G-quadruplex motif. A ready-to-use analytical device was developed by immobilizing GMS on the surface of a commercial screen-printed electrode, followed by the attachment of the aptamer. In the presence of Escherichia coli O157:H7, the binding sites of Hp with the G-quadruplex motif were opened and exposed to hemin, leading to the formation of a G-quadruplex/hemin DNAzyme. Crucially, after binding to hemin, the charge transfer pathway of GMS changes, resulting in a switch of the photocurrent polarity. Further, G-quadruplex/hemin DNAzyme enhanced the cathodic photocurrent, and the proposed sensor exhibited a wide linear range ((25.0-1.0) × 10 7 CFU/mL), a low limit of detection (2.0 CFU/mL), and good anti-interference performance. These findings expand the applications of NIR-responsive PEC materials and provide versatile PEC methods for detecting biological analytes, especially for food safety testing.
Keyphrases
- label free
- escherichia coli
- loop mediated isothermal amplification
- reduced graphene oxide
- sensitive detection
- human health
- gold nanoparticles
- gram negative
- high throughput
- quantum dots
- risk assessment
- photodynamic therapy
- real time pcr
- fluorescence imaging
- drug release
- high glucose
- cancer therapy
- drinking water
- multidrug resistant
- living cells
- drug delivery
- single molecule
- mass spectrometry