Real-time monitoring and model-based prediction of purity and quantity during a chromatographic capture of fibroblast growth factor 2.
Dominik Georg SauerMichael MelcherMagdalena MosorNicole WalchMatthias BerkemeyerTheresa Scharl-HirschFriedrich LeischAlois JungbauerAstrid DürauerPublished in: Biotechnology and bioengineering (2019)
Process analytical technology combines understanding and control of the process with real-time monitoring of critical quality and performance attributes. The goal is to ensure the quality of the final product. Currently, chromatographic processes in biopharmaceutical production are predominantly monitored with UV/Vis absorbance and a direct correlation with purity and quantity is limited. In this study, a chromatographic workstation was equipped with additional online sensors, such as multi-angle light scattering, refractive index, attenuated total reflection Fourier-transform infrared, and fluorescence spectroscopy. Models to predict quantity, host cell proteins (HCP), and double-stranded DNA (dsDNA) content simultaneously were developed and exemplified by a cation exchange capture step for fibroblast growth factor 2 expressed in Escherichia coliOnline data and corresponding offline data for product quantity and co-eluting impurities, such as dsDNA and HCP, were analyzed using boosted structured additive regression. Different sensor combinations were used to achieve the best prediction performance for each quality attribute. Quantity can be adequately predicted by applying a small predictor set of the typical chromatographic workstation sensor signals with a test error of 0.85 mg/ml (range in training data: 0.1-28 mg/ml). For HCP and dsDNA additional fluorescence and/or attenuated total reflection Fourier-transform infrared spectral information was important to achieve prediction errors of 200 (2-6579 ppm) and 340 ppm (8-3773 ppm), respectively.
Keyphrases
- single molecule
- simultaneous determination
- electronic health record
- big data
- high resolution
- quality improvement
- health information
- patient safety
- single cell
- healthcare
- social media
- optical coherence tomography
- magnetic resonance imaging
- machine learning
- computed tomography
- energy transfer
- adverse drug
- binding protein
- magnetic resonance
- liquid chromatography
- deep learning
- cell free
- dual energy