"Turn-on" Fluorescent Aptasensor Based on AIEgen Labeling for the Localization of IFN-γ in Live Cells.
Ke MaFengli ZhangNima SayyadiWenjie ChenAyad G AnwerAndrew CareBin XuWenjing TianEwa M GoldysGuozhen LiuPublished in: ACS sensors (2018)
We report an aggregation-induced emission fluorogen (AIEgen)-based turn-on fluorescent aptasensor able to detect the ultrasmall concentration of intracellular IFN-γ. The aptasensor consists of an IFN-γ aptamer labeled with a fluorogen with a typical aggregation-induced emission (AIE) characteristic, which shows strong red emission only in the presence of IFN-γ. The aptasensor is able to effectively monitor intracellular IFN-γ secretion with the lowest detection limit of 2 pg mL-1, and it is capable of localizing IFN-γ in live cells during secretion, with excellent cellular permeability and biocompatibility as well as low cytotoxicity. This probe is able to localize the intracellular IFN-γ at a low concentration <10 pg mL-1, and it is successfully used for real-time bioimaging. This simple and highly sensitive sensor may enable the exploration of cytokine pathways and their dynamic secretion process in the cellular environment. It provides a universal sensing platform for monitoring a spectrum of molecules secreted by cells.
Keyphrases
- quantum dots
- living cells
- label free
- sensitive detection
- dendritic cells
- immune response
- induced apoptosis
- fluorescent probe
- cell cycle arrest
- endoplasmic reticulum stress
- signaling pathway
- single molecule
- computed tomography
- energy transfer
- high resolution
- pi k akt
- high throughput
- single cell
- solid state
- iron oxide nanoparticles