The cap-binding complex modulates ABA-responsive transcript splicing during germination in barley (Hordeum vulgare).
Ewa SybilskaAnna CollinBahareh Sadat HaddadiLuis Alejandro Jose MurManfred BeckmannWenbin GuoCraig G SimpsonAgata Daszkowska-GolecPublished in: Scientific reports (2024)
To decipher the molecular bases governing seed germination, this study presents the pivotal role of the cap-binding complex (CBC), comprising CBP20 and CBP80, in modulating the inhibitory effects of abscisic acid (ABA) in barley. Using both single and double barley mutants in genes encoding the CBC, we revealed that the double mutant hvcbp20.ab/hvcbp80.b displays ABA insensitivity, in stark contrast to the hypersensitivity observed in single mutants during germination. Our comprehensive transcriptome and metabolome analysis not only identified significant alterations in gene expression and splicing patterns but also underscored the regulatory nexus among CBC, ABA, and brassinosteroid (BR) signaling pathways.
Keyphrases
- transcription factor
- arabidopsis thaliana
- gene expression
- dna binding
- signaling pathway
- genome wide
- rna seq
- single cell
- wild type
- genome wide identification
- dna methylation
- plant growth
- magnetic resonance
- binding protein
- computed tomography
- cancer therapy
- single molecule
- magnetic resonance imaging
- contrast enhanced
- drug induced
- drug delivery
- cell proliferation
- induced apoptosis