An OFF-ON Two-Photon Fluorescent Probe for Tracking Cell Senescence in Vivo.
Beatriz Lozano-TorresIrene GalianaMiguel RoviraEva GarridoSelim ChaibAndrea BernardosDaniel Muñoz-EspínManuel SerranoRamón Martínez-MáñezFélix SancenónPublished in: Journal of the American Chemical Society (2017)
A naphthalimide-based two-photon probe (AHGa) for the detection of cell senescence is designed. The probe contains a naphthalimide core, an l-histidine methyl ester linker, and an acetylated galactose bonded to one of the aromatic nitrogen atoms of the l-histidine through a hydrolyzable N-glycosidic bond. Probe AHGa is transformed into AH in senescent cells resulting in an enhanced fluorescent emission intensity. In vivo detection of senescence is validated in mice bearing tumor xenografts treated with senescence-inducing chemotherapy.
Keyphrases
- living cells
- fluorescent probe
- dna damage
- endothelial cells
- single cell
- stress induced
- cell therapy
- single molecule
- induced apoptosis
- loop mediated isothermal amplification
- label free
- stem cells
- quantum dots
- squamous cell carcinoma
- mesenchymal stem cells
- radiation therapy
- cell proliferation
- signaling pathway
- endoplasmic reticulum stress
- pi k akt