Login / Signup

Dimeric Metal-Salphen Complexes Which Target Multimeric G-Quadruplex DNA.

Timothy KenchViktoria RakersDavid BouzadaJacobo Gomez-GonzálezJenna RobinsonMarina K KuimovaMiguel Vázquez LópezM Eugenio VázquezRamon Vilar
Published in: Bioconjugate chemistry (2023)
G-Quadruplex DNA structures have attracted increasing attention due to their biological roles and potential as targets for the development of new drugs. While most guanine-rich sequences in the genome have the potential to form monomeric G-quadruplexes, certain sequences have enough guanine-tracks to give rise to multimeric quadruplexes. One of these sequences is the human telomere where tandem repeats of TTAGGG can lead to the formation of two or more adjacent G-quadruplexes. Herein we report on the modular synthesis via click chemistry of dimeric metal-salphen complexes (with Ni II and Pt II ) bridged by either polyether or peptide linkers. We show by circular dichroism (CD) spectroscopy that they generally have higher selectivity for dimeric vs monomeric G-quadruplexes. The emissive properties of the Pt II -salphen dimeric complexes have been used to study their interactions with monomeric and dimeric G-quadruplexes in vitro as well as to study their cellular uptake and localization.
Keyphrases
  • single molecule
  • high resolution
  • gene expression
  • endothelial cells
  • cell free
  • circulating tumor
  • dna methylation
  • solid state