A simple adaptation to a protein crystallography station to facilitate difference X-ray scattering studies.
Amit SharmaPeter BerntsenRajiv HarimoorthyRoberto AppioJennie SjöhamnMichael A JärvåAlexander BjörlingGreger HammarinSebastian WestenhoffGisela BrändénRichard NeutzePublished in: Journal of applied crystallography (2019)
The X-ray crystallography station I911-2 at MAXLab II (Lund, Sweden) has been adapted to enable difference small- and wide-angle X-ray scattering (SAXS/WAXS) data to be recorded. Modifications to the beamline included a customized flow cell, a motorized flow cell holder, a helium cone, a beam stop, a sample stage and a sample delivery system. This setup incorporated external devices such as infrared lasers, LEDs and reaction mixers to induce conformational changes in macromolecules. This platform was evaluated through proof-of-principle experiments capturing light-induced conformational changes in phytochromes. A difference WAXS signature of conformational changes in a plant aqua-porin was also demonstrated using caged calcium.
Keyphrases
- high resolution
- molecular dynamics
- molecular dynamics simulations
- single molecule
- single cell
- dual energy
- cell therapy
- electron microscopy
- magnetic resonance
- high throughput
- computed tomography
- stem cells
- magnetic resonance imaging
- big data
- mesenchymal stem cells
- mass spectrometry
- binding protein
- bone marrow
- deep learning
- data analysis
- cell wall