NIR-II Photoacoustic-Active DNA Origami Nanoantenna for Early Diagnosis and Smart Therapy of Acute Kidney Injury.
Yanan XuQian ZhangRunzhi ChenHaiting CaoJie TangYuqi WuXing LuBinbin ChuBin SongHouyu WangYao HePublished in: Journal of the American Chemical Society (2022)
Herein, we designed and synthesized a novel microRNA (miR)-responsive nanoantenna capable of early diagnosis and smart treatment of acute kidney injury (AKI). The nanoantenna was made of two miniature gold nanorods (AuNRs) ( e.g. , length: ∼48 nm; width: ∼9 nm) linked together by a rectangular DNA origami nanostructure (rDONs) scaffold ( e.g. , length: ∼90 nm; width: ∼60 nm) (rDONs@AuNR dimer). The surface plasmon resonance peak of the constructed nanoantenna is located within the NIR-II window ( e.g. , ∼1060 nm), thus guaranteeing photoacoustic (PA) imaging of the nanoantenna in deep tissues. Intriguingly, the nanoantenna displayed exclusive kidney retention in both healthy mice and ischemia reperfusion-induced AKI mice by leveraging the kidney-targeting ability of rDONs. Distinguished from the stable signals in the healthy mice, the PA signals of the nanoantenna would turn down in the AKI mice due to the AuNR detached from rDONs upon interaction with miR-21, which were up-expressed in AKI mice. The limit of detection toward miR-21 was down to 2.8 nM, enabling diagnosis of AKI as early as 10 min post-treatment with ischemia reperfusion, around 2 orders of magnitude earlier than most established probes. Moreover, the naked rDON scaffold generated by AKI could capture more reactive oxygen species ( e.g. , 1.5-fold more than rDONs@AuNR dimer), alleviating ischemic AKI. This strategy provided a new avenue for early diagnosis and smart treatment of AKI.
Keyphrases
- acute kidney injury
- cardiac surgery
- photodynamic therapy
- high fat diet induced
- fluorescence imaging
- cell proliferation
- long non coding rna
- reactive oxygen species
- long noncoding rna
- gene expression
- stem cells
- fluorescent probe
- bone marrow
- oxidative stress
- type diabetes
- high resolution
- living cells
- cell free
- insulin resistance
- metabolic syndrome
- wild type
- circulating tumor
- wastewater treatment
- drug release
- subarachnoid hemorrhage
- mass spectrometry
- cerebral ischemia
- silver nanoparticles