Anticancer Effects of Cold Atmospheric Plasma in Canine Osteosarcoma Cells.
Jaehak LeeHyunjin MoonBonghye KuKeunho LeeCheol-Yong HwangSeung-Joon BaekPublished in: International journal of molecular sciences (2020)
Osteosarcoma is known to be one of the frequently occurring cancers in dogs. Its prognosis is usually very poor, with a high incidence of lung metastasis. Although radiation therapy has become a major therapeutic choice for canine osteosarcoma, the high costs and unexpected side effects prevent some patients from considering this treatment. Cold atmospheric plasma (CAP) is an ionized gas with high energy at low temperatures, and it produces reactive oxygen species that mediate many signaling pathways. Although many researchers have used CAP as an anticancer therapeutic approach in humans, its importance has been neglected in veterinary medicine. In this study, D-17 and DSN canine osteosarcoma cell lines were treated with CAP to observe its anticancer activity. By high-content screening and flow cytometry, CAP-treated cells showed growth arrest and apoptosis induction. Moreover, the osteosarcoma cells exhibited reduced migration and invasion activity when treated with CAP. Overall, CAP exerted an anticancer effect on canine osteosarcoma cell lines. CAP may have the potential to be used as a novel modality for treating cancer in veterinary medicine.
Keyphrases
- cell cycle arrest
- induced apoptosis
- radiation therapy
- endoplasmic reticulum stress
- flow cytometry
- newly diagnosed
- cell death
- pi k akt
- signaling pathway
- reactive oxygen species
- oxidative stress
- particulate matter
- ejection fraction
- risk factors
- cell proliferation
- cell cycle
- air pollution
- human health
- lymph node metastasis