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A hybrid single cell demultiplexing strategy that increases both cell recovery rate and calling accuracy.

Lei LiJiayi SunYanbin FuSiriruk ChangrobJoshua J C McGrathPatrick C Wilson
Published in: bioRxiv : the preprint server for biology (2023)
An improved algorithm for cell hashing that distinguishes true positive from background for each individual hashtag at higher accuracyThis hybrid strategy increases cell recovery and calling accuracy while lowering experimental costThis hybrid demultiplexing strategy is applicable for single-cell RNA sequencing with different donor species, subjects, and cell populationsDoublet rate is a major determinant of the performance of SNP-based demultiplexing method.
Keyphrases
  • single cell
  • rna seq
  • high throughput
  • cell therapy
  • machine learning
  • gene expression
  • mesenchymal stem cells
  • dna methylation
  • deep learning