Efficient protein incorporation and release by a jigsaw-shaped self-assembling peptide hydrogel for injured brain regeneration.
Atsuya YaguchiMio OshikawaGo WatanabeHirotsugu HiramatsuNoriyuki UchidaChikako HaraNaoko KanekoKazunobu SawamotoTakahiro MuraokaItsuki AjiokaPublished in: Nature communications (2021)
During injured tissue regeneration, the extracellular matrix plays a key role in controlling and coordinating various cellular events by binding and releasing secreted proteins in addition to promoting cell adhesion. Herein, we develop a cell-adhesive fiber-forming peptide that mimics the jigsaw-shaped hydrophobic surface in the dovetail-packing motif of glycophorin A as an artificial extracellular matrix for regenerative therapy. We show that the jigsaw-shaped self-assembling peptide forms several-micrometer-long supramolecular nanofibers through a helix-to-strand transition to afford a hydrogel under physiological conditions and disperses homogeneously in the hydrogel. The molecular- and macro-scale supramolecular properties of the jigsaw-shaped self-assembling peptide hydrogel allow efficient incorporation and sustained release of vascular endothelial growth factor, and demonstrate cell transplantation-free regenerative therapeutic effects in a subacute-chronic phase mouse stroke model. This research highlights a therapeutic strategy for injured tissue regeneration using the jigsaw-shaped self-assembling peptide supramolecular hydrogel.
Keyphrases
- extracellular matrix
- stem cells
- tissue engineering
- wound healing
- cell therapy
- drug delivery
- vascular endothelial growth factor
- hyaluronic acid
- mesenchymal stem cells
- cell adhesion
- single cell
- atrial fibrillation
- energy transfer
- blood brain barrier
- water soluble
- resting state
- subarachnoid hemorrhage
- ionic liquid
- dna binding