Development of pENTR-NeCo-lacZα vectors for the preparation of negative control constructs in Gateway cloning.
Taiki KuzuharaKota MondenTakushi HachiyaTsuyoshi NakagawaPublished in: Bioscience, biotechnology, and biochemistry (2024)
Gateway cloning is a useful technology for the simple and reliable preparation of various construct in many organisms. However, there is a problem regarding the negative control construct in the Gateway cloning system. In this study, we developed the pENTR-NeCo-lacZα vector system to create an empty vector that can be used as a negative control construct in Gateway cloning.