Fluorescence-Amplified Origami Microneedle (FAOM) Device for Quantitatively Monitoring Blood Glucose.

Exploration of clinically acceptable blood glucose monitors has been engaging in the past decades, yet our ability to quantitatively detect blood glucose in a painless, accurate and highly sensitive manner remains limited. Herein, we describe a fluorescence-amplified origami microneedle (FAOM) device that integrates tubular DNA origami nanostructures and glucose oxidase molecules into its inner network to quantitatively monitor blood glucose. Skin-attached FAOM device can collect glucose in situ and transfer the input into proton signal with oxidase's catalysis. The proton-driven mechanical reconfiguration of DNA origami tubes separated fluorescent molecules and their quenchers, eventually amplifying glucose-correlated fluorescence signal. The function equation established on clinical examinees suggested that FAOM can report blood glucose in a highly sensitive and quantitative manner. In clinical blind tests, FAOM achieved well-matched accuracy (98.70 ± 4.77%) compared with commercial blood biochemical analyser and completely meets the requirements of accurately monitoring blood glucose. FAOM device could be inserted into skin tissue in a trivially painful manner and with minimal leakage of DNA origami, substantially improving the tolerance and compliance of blood glucose test. This article is protected by copyright. All rights reserved.