Extracellular Vesicles from a Biofilm of a Clinical Isolate of Candida albicans Negatively Impact on Klebsiella pneumoniae Adherence and Biofilm Formation.
Marianna ImparatoAngela MaioneAnnalisa BuonannoRenato GesueleNoemi GallucciMaria Michela CorsaroLuigi PaduanoAngela CasilloMarco GuidaEmilia GaldieroElisabetta de AlteriisPublished in: Antibiotics (Basel, Switzerland) (2024)
The opportunistic human fungal pathogen Candida albicans produces and releases into the surrounding medium extracellular vesicles (EVs), which are involved in some processes as communication between fungal cells and host-pathogen interactions during infection. Here, we have conducted the isolation of EVs produced by a clinical isolate of C. albicans during biofilm formation and proved their effect towards the ability of the Gram-negative bacterial pathogen Klebsiella pneumoniae to adhere to HaCaT cells and form a biofilm in vitro. The results represent the first evidence of an antagonistic action of fungal EVs against bacteria.
Keyphrases
- candida albicans
- biofilm formation
- klebsiella pneumoniae
- multidrug resistant
- gram negative
- induced apoptosis
- escherichia coli
- cell cycle arrest
- endothelial cells
- pseudomonas aeruginosa
- endoplasmic reticulum stress
- induced pluripotent stem cells
- weight loss
- metabolic syndrome
- cystic fibrosis
- adipose tissue
- insulin resistance
- cell wall