New insights into methotrexate accumulation in leukemia cells in vivo.

Published in: Molecular & cellular oncology (2021)

Measuring the amount of methotrexate polyglutamates (MTXPG) in leukemia cells after high-dose methotrexate (HDMTX) revealed that molecular subtype and lineage of acute lymphoblastic leukemia (ALL), the ratio of expression of folate influx and efflux transporters, methotrexate (MTX) infusion time, folylpolyglutamate synthase mRNA expression, and MTX systemic clearance explain 42% of the variation in active MTXPGs accumulation in ALL cells in vivo, providing insights into mechanisms underlying interpatient differences in the antileukemic effects of HDMTX.

Keyphrases

high dose
induced apoptosis
acute lymphoblastic leukemia
cell cycle arrest
low dose
acute myeloid leukemia
bone marrow
endoplasmic reticulum stress
cell death
oxidative stress
signaling pathway
long non coding rna
single molecule