Dynamic optical coherence tomography for cell analysis [Invited].
Salvatore AzzolliniTual MonfortOlivier ThouveninKate GrievePublished in: Biomedical optics express (2023)
Label-free live optical imaging of dynamic cellular and subcellular features has been made possible in recent years thanks to the advances made in optical imaging techniques, including dynamic optical coherence tomography (D-OCT) methods. These techniques analyze the temporal fluctuations of an optical signal associated with the active movements of intracellular organelles to obtain an ensemble metric recapitulating the motility and metabolic state of cells. They hence enable visualization of cells within compact, static environments and evaluate their physiology. These emerging microscopies show promise, in particular for the three-dimensional evaluation of live tissue samples such as freshly excised biopsies and 3D cell cultures. In this review, we compare the various techniques used for dynamic OCT. We give an overview of the range of applications currently being explored and discuss the future outlook and opportunities for the field.
Keyphrases
- optical coherence tomography
- high resolution
- induced apoptosis
- diabetic retinopathy
- label free
- cell cycle arrest
- single cell
- high speed
- optic nerve
- cell therapy
- oxidative stress
- escherichia coli
- machine learning
- cystic fibrosis
- big data
- reactive oxygen species
- pseudomonas aeruginosa
- staphylococcus aureus
- pi k akt
- convolutional neural network
- candida albicans