Assessing the Sensitivity of Nested PCR followed by direct sequencing on Exosomal DNA for EGFR Mutation Detection in NSCLC.
Mehdi JahaniParisa MashayekhiMir Davood OmraniAdnan KhosraviDehghanifard AliSanam Azad ManjiriMahyar ZahraeiMaryam MabaniSharareh SeifiBabak SalimiParsa RostamiPublished in: Iranian biomedical journal (2024)
Therapeutically targetable driver mutations in the EGFR gene can be accurately detected using nested PCR followed by direct sequencing of plasma exoDNA from patients with NSCLC. This approach facilitates timely and more personalized treatment for NSCLC patients, ultimately improving patient prognosis. Additionally, this method reduces the reliance on invasive tissue biopsies and their associated complications.
Keyphrases
- small cell lung cancer
- advanced non small cell lung cancer
- epidermal growth factor receptor
- end stage renal disease
- brain metastases
- tyrosine kinase
- ejection fraction
- real time pcr
- newly diagnosed
- chronic kidney disease
- single cell
- prognostic factors
- genome wide
- risk factors
- copy number
- patient reported outcomes
- transcription factor
- cell free
- label free
- ultrasound guided
- genome wide identification
- combination therapy