Mass Spectrometry-Based Detection of Beta Lactam Hydrolysis Enables Rapid Detection of Beta Lactamase Mediated Antibiotic Resistance.

Raymond T SuhandynataKyle LundAndrés M Caraballo-RodríguezSharon L ReedPieter C DorresteinRobert L FitzgeraldNicholas J Bevins

Published in: Laboratory medicine (2021)

Clinically significant beta lactamase activity is detectable directly from urine specimens. The described methods would enable the detection of beta lactam resistance 24 to 48 hours sooner than culture based methods.

Keyphrases

gram negative
mass spectrometry
escherichia coli
multidrug resistant
klebsiella pneumoniae
loop mediated isothermal amplification
real time pcr
liquid chromatography