Draft genome assembly of Passalora sequoiae a needle blight pathogen on Leyland cypress.

Warren E CopesJorge Ibarra CaballeroEbrahiem BabikerJane E StewartValerie A OrnerAlan S WindhamRenee S Arias

Published in: BMC research notes (2020)

We report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress 'Leighton Green' in 2017 in southern Mississippi, USA. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped to PacBio assembled contigs to determine base call consistency. Based on a total of 44 contigs with 722 kilobase (kb) average length (range 9.4 kb to 3.4 Mb), the whole genome size was estimated at 31,768,716 bp. Mapping of Illumina reads to PacBio contigs resulted in a 1000 × coverage and were used to confirm accuracy of the consensus sequences.

Keyphrases

high throughput sequencing
genome wide
high resolution
single cell
mass spectrometry
simultaneous determination
gene expression
dna methylation
affordable care act
high density