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Targeting the Retinoblastoma/E2F repressive complex by CDK4/6 inhibitors amplifies oncolytic potency of an oncolytic adenovirus.

Jana KochSebastian Johannes SchoberSruthi V HindupurCaroline SchöningFlorian Gerhard KleinKlaus MantwillMaximilian EhrenfeldUlrike SchillingerTimmy HohneckerPan QiKatja SteigerMichaela AichlerJürgen E GschwendRoman NawrothPer Sonne Holm
Published in: Nature communications (2022)
CDK4/6 inhibitors (CDK4/6i) and oncolytic viruses are promising therapeutic agents for the treatment of various cancers. As single agents, CDK4/6 inhibitors that are approved for the treatment of breast cancer in combination with endocrine therapy cause G1 cell cycle arrest, whereas adenoviruses induce progression into S-phase in infected cells as an integral part of the their life cycle. Both CDK4/6 inhibitors and adenovirus replication target the Retinoblastoma protein albeit for different purposes. Here we show that in combination CDK4/6 inhibitors potentiate the anti-tumor effect of the oncolytic adenovirus XVir-N-31 in bladder cancer and murine Ewing sarcoma xenograft models. This increase in oncolytic potency correlates with an increase in virus-producing cancer cells, enhanced viral genome replication, particle formation and consequently cancer cell killing. The molecular mechanism that regulates this response is fundamentally based on the reduction of Retinoblastoma protein expression levels by CDK4/6 inhibitors.
Keyphrases
  • cell cycle
  • cell cycle arrest
  • cell death
  • cell proliferation
  • sars cov
  • pi k akt
  • young adults
  • gene therapy
  • genome wide
  • signaling pathway
  • combination therapy
  • cell therapy
  • childhood cancer