Hyperglycemia Impairs Neutrophil-Mediated Bacterial Clearance in Mice Infected with the Lyme Disease Pathogen.
Ashkan JavidNataliya ZlotnikovHelena PětrošováTian Tian TangYang ZhangAnil K BansalRhodaba EbadyMaitry ParikhMijhgan AhmedChunxiang SunSusan NewbiggingYae Ram KimMarianna Santana SosaMichael GlogauerTara J MoriartyPublished in: PloS one (2016)
Insulin-insufficient type 1 diabetes is associated with attenuated bactericidal function of neutrophils, which are key mediators of innate immune responses to microbes as well as pathological inflammatory processes. Neutrophils are central to immune responses to the Lyme pathogen Borrelia burgdorferi. The effect of hyperglycemia on host susceptibility to and outcomes of B. burgdorferi infection has not been examined. The present study investigated the impact of sustained obesity-independent hyperglycemia in mice on bacterial clearance, inflammatory pathology and neutrophil responses to B. burgdorferi. Hyperglycemia was associated with reduced arthritis incidence but more widespread tissue colonization and reduced clearance of bacterial DNA in multiple tissues including brain, heart, liver, lung and knee joint. B. burgdorferi uptake and killing were impaired in neutrophils isolated from hyperglycemic mice. Thus, attenuated neutrophil function in insulin-insufficient hyperglycemia was associated with reduced B. burgdorferi clearance in target organs. These data suggest that investigating the effects of comorbid conditions such as diabetes on outcomes of B. burgdorferi infections in humans may be warranted.
Keyphrases
- type diabetes
- glycemic control
- high fat diet induced
- insulin resistance
- diabetic rats
- immune response
- innate immune
- oxidative stress
- cardiovascular disease
- candida albicans
- metabolic syndrome
- rheumatoid arthritis
- heart failure
- gene expression
- circulating tumor
- body mass index
- resting state
- risk factors
- dendritic cells
- skeletal muscle
- multiple sclerosis
- functional connectivity
- circulating tumor cells
- atomic force microscopy