Conformationally Induced Off-On Two-Photon Fluorescent Bioprobes for Dynamically Tracking the Interactions among Multiple Organelles.
Huihui ZhangXiaojiao ZhuGang LiuXinzhi DingJunjun WangMingdi YangRuilong ZhangZhongping ZhangYupeng TianHong-Ping ZhouPublished in: Analytical chemistry (2019)
Unveiling the synergism among multiple organelles for fully exploring the mysteries of the cell has drawn more and more attention. Herein, we developed two two-photon fluorescent bioprobes (Lyso-TA and Mito-QA), of which the conformational change triggered an "off-on" fluorescent response. Lyso-TA can real-time monitor the fusion and movement of lysosomes as well as unveil the mitophagy process with the engagement of lysosomes. Mito-QA was transformed from Lyso-TA by one-step ambient temperature reaction, visualizing the dysfunctional mitochondria through a shift from mitochondria to nucleoli. With superior two-photon absorption cross section, good biocompatibility, and greater penetration depth, two small bioprobes were both applied in in vivo bioimaging of brain tissues and zebrafish.
Keyphrases
- living cells
- fluorescent probe
- single molecule
- quantum dots
- cell death
- air pollution
- reactive oxygen species
- gene expression
- high glucose
- endoplasmic reticulum
- working memory
- single cell
- cell therapy
- particulate matter
- molecular dynamics simulations
- white matter
- social media
- molecular dynamics
- endothelial cells
- drug induced
- stem cells
- nlrp inflammasome