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Comparative Transcriptomic Analysis of Inarching Invigorating Rootstock onto Incompatible Grafts in Citrus.

Wen HeRui XieLiang LuoJiufeng ChaiHao WangYan WangQing ChenZhiwei WuShaofeng YangMengyao LiYuanxiu LinYunting ZhangYa LuoYunting ZhangHao-Ru TangFrederick G GmitterXiao-Rong Wang
Published in: International journal of molecular sciences (2022)
Grafting is a technique that is widely used in citrus production. Graft incompatibility often occurs in the orchard. Inarching can effectively improve the vigor of incompatible grafts, but its mechanism remains poorly understood. Our previous studies investigated the scion-rootstock interaction of citrus and highlighted the role of hormonal balance and genes in abscisic acid biosynthesis regulation. To further elucidate the mechanism of inarched grafts rejuvenation, Hm/Pt combination ( Citrus maxima (Burm.) Merrill cv. 'Hongmian miyou' grafted onto Poncirus trifoliata ) were inarched with 'Pujiang Xiangcheng' (a novel citrus rootstock cultivar recently selected from wild Citrus junos populations), and comprehensive analysis was performed to compare the inarched grafts and controls. Compared with incompatible grafts, the results revealed that inarching could recover the leaf metabolism balance, including reducing starch content, increasing chlorophyll content and restoring the cell structure. Additionally, our results corroborated that hormonal balance and hormone-related genes played a central role in inarching compatibility. Furthermore, the roles of Hsf4 , ERF1 , NCED3 and PYL were highlighted, and a model for explaining inarched grafts recovery invigoration was proposed. This study shed light on the mechanism of inarching regulation tree vigor and offered deep insights into the scion-rootstock interaction in citrus.
Keyphrases
  • single cell
  • transcription factor
  • stem cells
  • metabolic syndrome
  • dna methylation
  • bone marrow
  • mesenchymal stem cells
  • high resolution
  • polycystic ovary syndrome
  • bioinformatics analysis