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Biological and molecular characterization of a new potexvirus isolated from Adenium obesum.

Vinicius Henrique BelloTatsuya NagataJulio Massaharu MarubayashiRenate Krause-SakateLayla de Sá Andrade MedeirosJosé Evando Aguiar Beserra JúniorJorge Alberto Marques RezendeElliot Watanabe Kitajima
Published in: Archives of virology (2023)
Adenium obesum plants showing virus-like symptoms were collected in several regions of Brazil. Mottling symptoms like those observed in symptomatic plants in the field were reproduced in mechanically inoculated A. obesum plants. This potexvirus was named "desert rose mottle virus" (DRMoV), and its genome sequence was first determined by high-throughput sequencing and then confirmed by Sanger sequencing. The complete genome of DRMoV is 6,781 nt in length, excluding the poly(A) tail, and five ORFs were predicted in order from 5' to 3': Rep-TGB1-TGB2-TGB3-CP. Phylogenetic analysis based on Rep amino acid sequences showed different clustering among potexviruses. These data suggest that RDMoV is a new member of the genus Potexvirus, and the binomial name "Potexvirus adenii" is proposed for its species.
Keyphrases
  • amino acid
  • high throughput sequencing
  • single cell
  • genome wide
  • sleep quality
  • electronic health record
  • big data
  • genetic diversity
  • gene expression
  • depressive symptoms
  • artificial intelligence