Electrophoresis and Biosensor-Based DNA Interaction Analysis of the First Paraben Derivatives of Spermine-Bridged Cyclotriphosphazenes.
Elif ŞenkuytuPerihan KızılkayaZehra ÖlçerUğur PalaDerya DavarcıYunus ZorluHuriye ErdoğanGönül Yenilmez ÇiftçiPublished in: Inorganic chemistry (2020)
Cancer is the uncontrolled growth of abnormal cells via malignant cell division and rapid DNA replication. While DNA damaging molecules can cause cancer, their role as anticancer drugs are very significant. For this purpose, the novel series of paraben substituted spermine bridged(dispirobino) cyclotriphosphazene compounds 2-6 were synthesized for the first time, and their structures were characterized by various spectroscopic techniques. The solid-state structures and geometries of compounds 2-6 were determined using single-crystal X-ray structural analysis. In addition, it was confirmed by TGA that all compounds 1-6 showed high thermal stability. Two methods were used in order to investigate DNA interaction properties of the targeted molecules. While biosensor-based screening test that measures DNA hybridization efficiency on a biochip surface, the agarose gel electrophoresis method examines the effect of compounds on plasmid DNA structure. The results collected from the automated biosensor device and agarose gel electrophoresis have indicated that compounds 1, 5, and 6 showed higher DNA damage than the compounds 2-4. According to the biosensor results, compounds 1, 5, and 6 showed 85%, 69%, and 77% activity, respectively.
Keyphrases
- circulating tumor
- single molecule
- cell free
- dna damage
- gold nanoparticles
- sensitive detection
- quantum dots
- nucleic acid
- solid state
- label free
- high resolution
- papillary thyroid
- escherichia coli
- molecular docking
- oxidative stress
- machine learning
- single cell
- induced apoptosis
- squamous cell carcinoma
- crispr cas
- magnetic resonance
- cell death
- computed tomography
- dna repair
- high throughput
- mesenchymal stem cells
- pi k akt
- childhood cancer