Optimal pH shift of the NADH oxidase from Lactobacillus rhamnosus with a single mutation.

Published in: Biotechnology letters (2021)

The D251R mutant displayed higher catalytic activity than the wild-type in the pH range 6.5-7.5, and further insight into those shorter and newly formed hydrogen bonds in substrate docking analysis could account for the higher bind affinity and catalytic efficiency of D251R mutant.

Keyphrases

wild type
molecular dynamics
molecular dynamics simulations
protein protein
small molecule
mass spectrometry