Serum Autoantibody Biomarkers for Management of Rheumatoid Arthritis Disease.
Esther Sánchez-TiradoLourdes AgüíMarta Sánchez-PaniaguaAraceli González-CortésBeatriz López-RuizPaloma Yañez-SedeñoJosé Manuel PingarrónPublished in: Biosensors (2023)
Rheumatoid arthritis (RA) is a systemic chronic autoimmune inflammatory disease that is characterized by the destruction of bone and production of autoantibodies such as rheumatoid factor (RF) and anticitrullinated protein antibodies (ACPAs). The high prevalence of this disease and the need of affordable tools for its early detection led us to prepare the first electrochemical immunoplatform for the simultaneous determination of four RA biomarkers, the autoantibodies: RF, anti-peptidyl-arginine deiminase enzyme (anti-PAD4), anti-cyclic citrullinated peptide (anti-CCP), and anti-citrullinated vimentin (anti-MCV). Functionalized magnetic beads (MBs) were used to immobilize the specific antigens, and sandwich-type immunoassays were implemented for the amperometric detection of the four autoantibodies, using the horseradish peroxidase (HRP)/H 2 O 2 /hydroquinone (HQ) system. The immunoplatform was applied to the determination of the biomarkers in human serum of twenty-two patients diagnosed with RA and four healthy individuals, and the results were validated against ELISA tests and the certified values.
Keyphrases
- rheumatoid arthritis
- simultaneous determination
- systemic lupus erythematosus
- disease activity
- molecularly imprinted
- interstitial lung disease
- high resolution
- liquid chromatography tandem mass spectrometry
- hydrogen peroxide
- nitric oxide
- immune response
- end stage renal disease
- high performance liquid chromatography
- ms ms
- bone mineral density
- systemic sclerosis
- body composition
- mass spectrometry
- drug induced
- soft tissue
- patient reported