Beamline B21: high-throughput small-angle X-ray scattering at Diamond Light Source.
Nathan Philip CowiesonCharlotte J C Edwards-GayleKatsuaki InoueNikul S KhuntiJames DoutchEugene WilliamsSteven DanielsGeoff PreeceNicholas A KrumpaJohn P SutterMark D TullyNicholas J TerrillRobert P RamboPublished in: Journal of synchrotron radiation (2020)
B21 is a small-angle X-ray scattering (SAXS) beamline with a bending magnet source in the 3 GeV storage ring at the Diamond Light Source Ltd synchrotron in the UK. The beamline utilizes a double multi-layer monochromator and a toroidal focusing optic to deliver 2 × 1012 photons per second to a 34 × 40 µm (FWHM) focal spot at the in-vacuum Eiger 4M (Dectris) detector. A high-performance liquid chromatography system and a liquid-handling robot make it possible to load solution samples into a temperature-controlled in-vacuum sample cell with a high level of automation. Alternatively, a range of viscous or solid materials may be loaded manually using a range of custom sample cells. A default scattering vector range from 0.0026 to 0.34 Å-1 and low instrument background make B21 convenient for measuring a wide range of biological macromolecules. The beamline has run a full user programme since 2013.
Keyphrases
- high resolution
- high performance liquid chromatography
- high throughput
- single cell
- mass spectrometry
- tandem mass spectrometry
- simultaneous determination
- cell therapy
- solid phase extraction
- functional connectivity
- dual energy
- ionic liquid
- computed tomography
- optical coherence tomography
- resting state
- cancer therapy
- signaling pathway
- magnetic resonance
- study protocol
- cell proliferation
- optic nerve