Asymmetric Monomethine Cyanine Dyes with Hydrophobic Functionalities for Fluorescent Intercalator Displacement Assay.
Sonia IlievaNadezhda BozovaMiroslav RangelovNadezhda TodorovaAleksey A VasilevDiana Valentinova CheshmedzhievaPublished in: Molecules (Basel, Switzerland) (2023)
A new green procedure has been applied for the synthesis and purification of asymmetric monomethine cyanine dyes. The photophysical properties of the newly synthesized compounds have been examined by combined application of spectroscopic and theoretical methods. The structural characteristics of the molecules and dimer formation were characterized by quantum chemical computation and juxtaposed to the aggregachromism in UV/Vis spectra. The applicability of the dyes as fluorogenic nucleic acid probes has been proven by fluorescence titration, and their binding constants have been calculated. The mode of ligand-dsDNA/RNA interaction was rationalized by means of CD spectroscopy, molecular docking analysis, and fluorescent intercalator displacement experiments.
Keyphrases
- molecular docking
- nucleic acid
- aqueous solution
- living cells
- single molecule
- quantum dots
- molecular dynamics simulations
- solid state
- energy transfer
- fluorescent probe
- molecular dynamics
- high resolution
- high throughput
- minimally invasive
- density functional theory
- ionic liquid
- binding protein
- dna binding
- single cell
- mass spectrometry
- nk cells