Simultaneous Detection of Carbon Monoxide and Viscosity Changes in Cells.
Jonathan A RobsonMarkéta KubánkováTamzin BondRian A HendleyAndrew J P WhiteMarina K KuimovaJames D E T Wilton-ElyPublished in: Angewandte Chemie (International ed. in English) (2020)
A new family of robust, non-toxic, water-compatible ruthenium(II) vinyl probes allows the rapid, selective and sensitive detection of endogenous carbon monoxide (CO) in live mammalian cells under normoxic and hypoxic conditions. Uniquely, these probes incorporate a viscosity-sensitive BODIPY fluorophore that allows the measurement of microscopic viscosity in live cells via fluorescence lifetime imaging microscopy (FLIM) while also monitoring CO levels. This is the first example of a probe that can simultaneously detect CO alongside small viscosity changes in organelles of live cells.
Keyphrases
- induced apoptosis
- sensitive detection
- cell cycle arrest
- single molecule
- living cells
- high resolution
- loop mediated isothermal amplification
- small molecule
- endoplasmic reticulum stress
- quantum dots
- oxidative stress
- signaling pathway
- cell death
- cell proliferation
- mass spectrometry
- pi k akt
- optical coherence tomography
- label free
- high speed