Prider: multiplexed primer design using linearly scaling approximation of set coverage.
Niina SmolanderTimothy R JulianManu TamminenPublished in: BMC bioinformatics (2022)
Prider solves a recalcitrant problem in molecular diagnostics: how to cover a maximal sequence diversity with a minimal number of oligonucleotide primers or probes. The combination of Prider with highly scalable molecular quantification techniques will permit an unprecedented molecular screening capability with immediate applicability in fields such as clinical microbiology, epidemic virus surveillance or antimicrobial resistance surveillance.