BZ Junctions and Its Application as Probe (2AP) to Detect Z-DNA Formation and Its Effector.
MinSoung KangDoyoun KimPublished in: Methods in molecular biology (Clifton, N.J.) (2023)
The left-handed Z-DNA is surrounded by right-handed canonical B-DNA, and thus the junction between B- and Z-DNA has been occurred during temporal Z-DNA formation in the genome. The base extrusion structure of the BZ junction may help detect Z-DNA formation in DNAs. Here we describe the BZ junction structural detection by using 2-aminopurine (2AP) fluorescent probe. BZ junction formation can be measured in solution by this method.